Novel Detection Methods in Food Safety

This topic was moderated by Hau-Yang Tsen, Ph.D. and Vivian Chi-Hua Wu, Ph.D. and featured five lectures.

 

Chien-Shun Chiou, Ph.D., Principal Investigator, Head of Central Regional Laboratory, Center for Research and Diagnostics, Centers for Disease Control, Taiwan discussed the development and application of molecular subtyping methods to surveil Salmonellosis in Taiwan, and shared findings that provided insight and understanding into an international salmonellosis outbreak through infant milk formula and accumulated large amount of PFGE and AST data that are useful in tracking the principal reservoirs for human salmonellosis and predicting Salmonella serotypes. Through the comparison of PFGE patterns with those in the database, findings indicated that pig is a principal reservoir for human salmonellosis in Taiwan and successfully predicted the serotypes for 99.4% of 862 Salmonella isolates collected in 2012.

 

Yao-Wen Huang, Ph.D., Professor, University of Georgia, USA discussed rapid detection of pathogens and toxins in the food system using surface-enhanced Raman spectroscopy (SERS) with silver nanorod array substrates. Dr. Huang noted that the use of aligned silver nanorod (AgNR) arrays prepared by oblique angle deposition (OAD), as surface-enhanced Raman scattering (SERS) substrates to detect food safety related substances such as foodborne pathogens, toxins, pesticides, and adulteration agents. Six foodborne pathogenic bacteria including Salmonella enterica serotype Anatum, S. enterica serotype Cubana, S. enterica serotype Stanley, S. enteritidis, E. coli O157:H7 and S. epidermidis in mung bean sprouts were detected using functionalized substrates with vancomycin. Dr. Huang shared that the results show bacterial species and serotypes can be detected and differentiated at the limit of 100cfu/ml within four hours.

 

Vivian Chi-Hua Wu, Ph.D., Associate Professor, Department of Food Science & Human Nutrition, University of Maine, USA discussed pathogen detection using biosensors and nanotechnology, and noted that a variety of rapid methods have been developed to detect and screen foodborne pathogens. These methods include antibody-based methods, such as immunofluorescence, immunoimmobilization, enzyme-linked immunosorbent assay, immunomagnetic separation, and nucleic acid-based methods such as hybridization and polymerase chain reaction [PCR], as well as biochemical and enzymatic methods such as miniaturized microbiological methods and commercial miniaturized diagnostic kits.

 

Hua-Yang Tsen, Ph.D., Professor and Dean, College of Human Ecology, Hungkuang University, Taiwan gave a presentation entitled, “Development and Use of Molecular Methods for the Detection of Food Pathogens, Virus, and Biological Species in Foods.” Dr. Tsen discussed various molecular detection techniques, such as polymerase chain reaction (PCR), multiplex PCR, DNA probes and biochips, real-time PCR (Q PCR), reverse transcription (RT) Q PCR, and insulated isothermal PCR (ii PCR), specific for the detection of different bacteria or biological species. Dr. Tsen noted that certain techniques have been established in order to trace the contamination origins of pathogenic strains and to conduct epidemiological study, including genotyping techniques such as PFGE, RAPD, MLST.

 

Tzann-Feng Lin, Ph.D., President, TTL, Taipei, Taiwan discussed systematic quality detection for functional foods, using the example of Monascus products. Dr. Lin shared study conclusions that Monascus TTL-123 is a suitable source ingredient for the production of Monascus health products, and emphasized that standardized safety analysis methods and understanding of functionality and beneficial effects are required for quality control of functional food products.

 

Food Contamination

This topic was moderated by Cheng-Ming Chang, Ph.D. and Yi-Chen Chen, Ph.D. and featured four lectures.

 

Ching-Cheng Chang, Ph.D., Professor, Department of Agricultural Economics, National Taiwan University, Taiwan gave a presentation entitled, “The Emerging Food Contamination Issues and Policy Implications in Asia-Pacific Region.” Dr. Chang emphasized that regional collaboration and information sharing between countries in the Asia Pacific is essential to preventing food contamination and mitigating food safety risks, especially in light of recent food safety challenges in various Asia Pacific countries.

 

Alexander Mathys, Ph.D., Head of Bioeconomy, The German Institute of Food Technologies, Germany discussed bacterial spore control by innovative multi-hurdle technology concepts. Dr. Mathys stated that new processes need to be developed to minimize the impact of the intensity of food processing on the quality of food products. Dr. Mathys suggested that these solutions be combined with assessments of physical and chemical challenges in order to promote adequate bacterial spore inactivation or inhibition during the shelf storage period for processed foods. This innovative concept utilizing multi-hurdle technology can greatly help to control bacterial spore contamination and ensure safe processed foods.

 

Alexander Panin, Ph.D., Director, The All-Russian State Center for Quality and Standardization of Veterinary Drugs and Feed, Moscow, Russia discussed food security and food safety and the role of veterinary services. Dr. Panin discussed the growing global population and predicted that due to this increase, there will be a 50% increase in consumption of animal protein by the year 2020. In order to meet this growing demand, Dr. Panin highlighted the importance of developing new approaches to control the safety of animals consumed for food, in order to promote food security and ensure a safe global food supply.

 

Jae-Hyung Mah, Ph.D., Associate Professor and Chair, Department of Food and Biotechnology, Korea University, Korea discussed the safety of fermented soybean products and the occurrence of biogenic amines. Biogenic amines are nitrogenous compounds produced by microbial amino acids in various foods which may cause disease in humans. Dr. Mah addressed possible situations in which biogenic amines can accumulate in fermented foods, and noted that fermented soybeans particularly can contain abundant dietary amino acid precursors of biogenic amines, though most often this contamination is within acceptable levels.

 

Investigation into dioxins in eggs is to focus on feed

Source: Taipei Times (http://www.taipeitimes.com/News/taiwan/archives/2017/05/02/2003669810)

Staff writer, with CNA

egg

An investigation into dioxins that contaminated eggs from a Changhua County chicken farm is focusing on chicken feed after ruling out water, air and soil as the source of contamination, the Council of Agriculture (COA) said on Sunday.

The Hung Chang Chicken Farm last week supplied dioxin-tainted eggs to a Miaoli County store, council officials said.

The farm changed management two months ago, the council said, adding that the family that runs the farm had been making the feed.

On Friday, more than 42,000 chickens were culled and incinerated by the county’s Department of Agriculture and Animal Disease Control Center, as well as about 15 tonnes of eggs.

Meanwhile, of the 8,962kg of eggs that were removed from store shelves as a precautionary measure, 3,730kg were free of dioxins and have been released for sale. The rest are to be destroyed, the Food and Drug Administration said on Friday.

Tests found 2.88 picogram/gram (pg/g) and 3.34pg/g of dioxin in two eggs from Hung Chang farm, above the permissible level of 2.5pg/g. One picogram is one trillionth of a gram.

Dioxins, a group of highly toxic and potentially carcinogenic chemicals, are pollutants produced as a result of human activity, such as burning garbage, and can accumulate in the food chain, mainly in the fatty tissue of animals.

Dioxins can damage the immune system, interfere with hormones and can cause cancer, reproductive and developmental problems, according to the WHO.

On April 21, the FDA ordered eggs from three farms in Changhua County’s Fangyuan Township (芳苑) be removed from store shelves as a precautionary measure after excessive levels of dioxins were found in an egg from the area.

After investigation, two of the three farms were cleared and Hung Chang farm was identified as the source of the problem.

The owners of the other two farms are demanding government compensation for loss of revenue.

 

NCFSER NEWS »

Novel Detection Methods in Food Safety

This topic was moderated by Hau-Yang Tsen, Ph.D. and Vivian Chi-Hua Wu, Ph.D. and featured five lectures.   Chien-Shun Chiou, Ph.D., Principal Investigator,...

 

Food Contamination

This topic was moderated by Cheng-Ming Chang, Ph.D. and Yi-Chen Chen, Ph.D. and featured four lectures.   Ching-Cheng Chang, Ph.D., Professor, Department of...

 
Investigation into dioxins in eggs is to focus on feed

Investigation into dioxins in eggs is to focus on feed

Source: Taipei Times (http://www.taipeitimes.com/News/taiwan/archives/2017/05/02/2003669810) Staff writer, with CNA An investigation into dioxins that contaminated...

 
TAFP held 2017 Annual Meeting & Dietary Supplement Forum on March 30

TAFP held 2017 Annual Meeting & Dietary Supplement Forum on March 30

Taiwan Association for Food Protection (TAFP) is the 48th international branch of the International Association for Food Protection (IAFP). TAFP opened the annual...